MXD/MIZ1 transcription regulatory complexes activate the expression of Myc ‐repressed genes

We report that MXDs activate transcription ofp15 andp21 in U2OS cells. This activation required DNA binding by MXDs and their interaction with MIZ1. MXD mutants deficient in MIZ1 ‐binding interacted with the Myc‐binding partner MAX and were active as repressors of MYC‐activated genes but failed to activate MYC‐repressed genes. Mutant MXDs with reduced DNA binding affinity interacted with MAX and MIZ1 but neither repressed nor activated transcription. Our data show th at MXDs and MYC have a reciprocally antagonistic potential to regulate transcription of target genes.

Source: FEBS Letters - April 29, 2021 Category: Biochemistry Authors: Anton Shostak, G éza Schermann, Axel Diernfellner, Michael Brunner Tags: RESEARCH ARTICLE Source Type: research