Primary human mesothelial cell culture in the evaluation of the inflammatory response to different sclerosing agents used for pleurodesis

We performed the prospective study using primary, biopsy ‐derived human mesothelial cells to establish an in vitro culture and to assess the response of pleural mesothelial cells to four different sclerosing agents (talc, povidone‐iodine, doxycycline, and transforming growth factor β) in different doses. Our study shows, that it is feasible to cultur e pleural mesothelial cells isolated from pleura biopsy specimens, that can be used for research on pleurodesis mechanisms. We found that pro‐inflammatory mesothelial response includes an increase in interleukin (IL)‐1β mRNA expression and protein production, which suggests the role of IL‐1β in the formation and maintenance of the inflammatory response during pleurodesis.​ AbstractThe mechanisms of chemical pleurodesis are still not fully explained. We aimed to evaluate the feasibility of using primary biopsy ‐derived human mesothelial cells to establish an in vitro culture and to assess the response of pleural mesothelial cells to different sclerosing agents. Talc, povidone‐iodine, doxycycline, and TGF‐β were used at different doses to stimulate pleural mesothelial cells. After 6 and 24 h, mRNA expression of interleukin (IL)‐1β, IL‐6, IL‐8, TGF‐β, MCP‐1, IL‐17A, and MMP9 was measured in cultured cells, and the protein level of IL‐1β, IL‐6, and IL‐8 was measured in the culture supernatant. The most pronounced response was observed after talc exposure. It was expressed a s an increase in...
Source: Physiological Reports - Category: Physiology Authors: Tags: ORIGINAL ARTICLE Source Type: research