Viruses, Vol. 13, Pages 749: From Multiplex Serology to Serolomics —A Novel Approach to the Antibody Response Against the SARS-CoV-2 Proteome

Viruses, Vol. 13, Pages 749: From Multiplex Serology to Serolomics—A Novel Approach to the Antibody Response Against the SARS-CoV-2 Proteome Viruses doi: 10.3390/v13050749 Authors: Julia Butt Rajagopal Murugan Theresa Hippchen Sylvia Olberg Monique van Straaten Hedda Wardemann Erec Stebbins Hans-Georg Kräusslich Ralf Bartenschlager Hermann Brenner Vibor Laketa Ben Schöttker Barbara Müller Uta Merle Tim Waterboer The emerging SARS-CoV-2 pandemic entails an urgent need for specific and sensitive high-throughput serological assays to assess SARS-CoV-2 epidemiology. We, therefore, aimed at developing a fluorescent-bead based SARS-CoV-2 multiplex serology assay for detection of antibody responses to the SARS-CoV-2 proteome. Proteins of the SARS-CoV-2 proteome and protein N of SARS-CoV-1 and common cold Coronaviruses (ccCoVs) were recombinantly expressed in E. coli or HEK293 cells. Assay performance was assessed in a COVID-19 case cohort (n = 48 hospitalized patients from Heidelberg) as well as n = 85 age- and sex-matched pre-pandemic controls from the ESTHER study. Assay validation included comparison with home-made immunofluorescence and commercial enzyme-linked immunosorbent (ELISA) assays. A sensitivity of 100% (95% CI: 86–100%) was achieved in COVID-19 patients 14 days post symptom onset with dual sero-positivity to SARS-CoV-2 N and the receptor-binding domain of the spike protein. The specificity obtained with thi...
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This report describes how the authors adapted the research methodologies to continue work throughout the pandemic, despite various restrictions. The original studies had very high participation rates because of several methodological approaches that minimized barriers, especially in-person data collection. During the pandemic, distancing requirements precluded replication of these same methods. Research strategies that enabled staff participation during working hours, with designated time allotted for participation, was key for ensuring high participation rates, as access to technology, availability of free time and oth...
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I’m in the northeast, and it’s becoming a pain in the a** to eat anywhere. Everywhere is full, have to book a reservation way ahead to get anything. Many restaraunts have started to hold a credit card on file for reservation and charge fees for cancelation, seems to have made it better. Is this how it was pretty pandemic, I can’t remember? Is it this annoying other parts of the country? I will also add, I only sit down at a restaraunt with my spouse maybe once or twice a month.
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New York has announced three more cases of the omicron variant of the coronavirus, bringing to eight the number of state cases linked to the new variant
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If we keep allowing this virus to spread through unvaccinated populations, the next variant could be even more deadlyCoronavirus – latest updatesSee all our coronavirus coverageIt ’s almost two years since we first heard of Covid-19, and a year since the first Covid vaccines were rolled out. Yet this staggering progress is being squandered. We have drifted for months now, with richer countries, taking a very blinkered domestic focus, lulled into thinking that the worst of t he pandemic was behind us. This variant reminds us all that we remain closer to the start of the pandemic than the end.There is a lot we ne...
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Sir Jeremy Farrar, director of Wellcome Trust, suggests emergence of Omicron variant means pandemic is far from overCoronavirus – latest updatesSee all our coronavirus coverageThe emergence of the Omicron variant shows that the world is “closer to the start of the pandemic than the end”, one of Britain’s most senior scientific figures has warned, as he lamented a lack of political leadership over Covid.Sir Jeremy Farrar, the director of the Wellcome Trust whostepped down as a government scientific adviser last month, said the progress in combatting Covid-19 since its emergence was “being squan...
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Christmas parties Johnson’s staff held last year broke coronavirus restrictions.
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The omicron coronavirus variant has been found in 12 U.S. states just three days days after the first case in the country was announced.
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[Thomson Reuters Foundation] Developing countries want the right to make their own COVID-19 vaccines, but opponents say there are better ways to boost supplies
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