Viruses, Vol. 13, Pages 698: Replication-Competent ΔNS1 Influenza A Viruses Expressing Reporter Genes
In this report, we describe, for the first time, the generation and characterization of two replication-competent influenza A/Puerto Rico/8/1934 H1N1 (PR8) viruses where the viral non-structural protein 1 (NS1) was substituted by the monomeric (m)Cherry fluorescent or the NanoLuc luciferase (Nluc) proteins. The ΔNS1 mCherry was able to replicate in cultured cells and in Signal Transducer and Activator of Transcription 1 (STAT1) deficient mice, although at a lower extent than a wild-type (WT) PR8 virus expressing the same mCherry fluorescent protein (WT mCherry). Notably, expression of either reporter gene (mCherry or Nluc) was detected in infected cells by fluorescent microscopy or luciferase plate readers, respectively. ΔNS1 IAV expressing reporter genes provide a novel approach to better understand the biology and pathogenesis of IAV, and represent an excellent tool to develop new therapeutic approaches against IAV infections.
Source: Viruses - Category: Virology Authors: Aitor Nogales Michael Schotsaert Raveen Rathnasinghe Marta L. DeDiego Adolfo Garc ía-Sastre Luis Martinez-Sobrido Tags: Article Source Type: research
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