Complement Detection in Human Tumors by Immunohistochemistry and Immunofluorescence

Methods Mol Biol. 2021;2227:191-203. doi: 10.1007/978-1-0716-1016-9_18.ABSTRACTTumors contain a complement rich microenvironment in which all cell types (e.g., tumor cells and stromal cells) are able to produce different proteins. We developed immunohistochemistry (IHC) assays allowing to identify on paraffin embedded tumor sections, not only the complement producing cells but also the complement activation fragments which result from activation of complement cascade within the tumor. The local production of complement can be detected by cytoplasmic staining, whereas the activation fragments are localized at the surface of the cells. There is a high heterogeneity of the staining within tumors but also between patients. Semi-quantification of the staining in large cohorts of patients allows to investigate the prognostic impact of the local complement production and activation. Here we explain the staining process for C1q, C4, and C3 in human paraffin-embedded tumor sections by immunofluorescence and immunohistochemistry.PMID:33847943 | DOI:10.1007/978-1-0716-1016-9_18
Source: Mol Biol Cell - Category: Molecular Biology Authors: Source Type: research