Optical Tweezers to Investigate the Structure and Energetics of Single-Stranded DNA-Binding Protein-DNA Complexes

Methods Mol Biol. 2021;2281:273-288. doi: 10.1007/978-1-0716-1290-3_17.ABSTRACTOptical tweezers enable the isolation and mechanical manipulation of individual nucleoprotein complexes. Here, we describe how to use this technique to interrogate the mechanical properties of individual protein-DNA complexes and extract information about their overall structural organization.PMID:33847965 | DOI:10.1007/978-1-0716-1290-3_17

Source: Mol Biol Cell - April 13, 2021 Category: Molecular Biology Authors: Jos é A Morin Fernando Cerr ón Francisco J Cao-Garc ía Borja Ibarra Source Type: research