Construction of recombinant Escherichia coli for production of l -phenylalanine-derived compounds

Abstractl-phenylalanine is an important amino acid that is widely used in the fields of food flavors and pharmaceuticals. Apart froml-phenylalanine itself, various commercially valuable chemical compounds can also be generated via thel-phenylalanine biosynthesis pathway. Compared with direct extraction from plants or synthesis by chemical reaction, microbial production ofl-phenylalanine -derived compounds can overcome the drawbacks of environmental pollution, low yield, and mixtures of stereoisomeric products. Accordingly, increasing intracellular levels of precursors, deregulating feedback inhibition and transcription repression, engineering global regulators and other effective strategies have been implemented to produce differentl-phenylalanine -derived compounds in the excellent chassis hostEscherichia coli. Finally, this review highlights principal strategies for improving the production ofl-phenylalanine and/or its derivatives inE. coli, and discusses the future outlook for further enhancing the titer and yields of these compounds.
Source: World Journal of Microbiology and Biotechnology - Category: Microbiology Source Type: research