MicroRNA-34a regulates 5-HT2C expression in Dorsal Raphe and contributes to the anti-depressant-like effect of fluoxetine

In this study, acute and chronic fluoxetine treatment differentially alters the expression of 5-HT2C and microRNA-34a in the dorsal raphe. Moreover, by in vitro luciferase assay, we demonstrated the repressive regulatory activity of microRNA-34a against 5-HT2C mRNA. Specific blockade of this interaction through local infusion of a target site blocker was sufficient to prevent the behavioral effects of chronic fluoxetine. Our results demonstrate a new miR-34a-mediated regulatory mechanism of 5-HT2C expression in the dorsal raphe and implicate it in eliciting the behavioral responses to chronic fluoxetine treatment.PMID:33845072 | DOI:10.1016/j.neuropharm.2021.108559

Source: Neuropharmacology - April 12, 2021 Category: Drugs & Pharmacology Authors: Luisa Lo Iacono Donald Ielpo Chiara Parisi Giulia Napoli Alessandra Accoto Matteo Di Segni Lucy Babicola Sebastian Luca D'Addario Serafina Manila Guzzo Tiziana Pascucci Rossella Ventura Diego Andolina Source Type: research