An Automated Correction Algorithm (ALPACA) for ddPCR Data Using Adaptive Limit of Blank and Correction of False Positive Events Improves Specificity of Mutation Detection

CONCLUSIONS: Correction of PIFs and application of an adaptive LoB increases specificity without a loss of sensitivity in ddPCR, leading to a higher accuracy in a real-life cohort of patients with stage IV NSCLC.PMID:33842952 | DOI:10.1093/clinchem/hvab040

Source: Clinical Lung Cancer - April 12, 2021 Category: Cancer & Oncology Authors: Daan C L Vessies Theodora C Linders Mirthe Lanfermeijer Kalpana L Ramkisoensing Vincent van der Noort Robert D Schouten Gerrit A Meijer Michel M van den Heuvel Kim Monkhorst Daan van den Broek Source Type: research