Determination of antimicrobial resistance profiles and mechanisms of < em > Campylobacter jejuni < /em > using a Raman spectroscopy-based metabolomic approach

In this study, we have applied Raman spectroscopy and chemometrics to achieve a rapid, accurate, and easy-to-operate investigation on bacterial AMR profiles and mechanisms. Raman spectroscopy reduced the analysis time by an order of magnitude to obtain the same results from traditional culture-based antimicrobial susceptibility approaches. It offers great benefits as a high-throughput screening method in food chain surveillance and clinical diagnostics. Meanwhile, the AMR mechanisms towards two representative antibiotic classes, namely ampicillin and tetracycline, were revealed by Raman spectroscopy at metabolome level. This approach is based on bacterial phenotypic responses to antibiotics, providing complementary information to the conventional genetic methods such as genome sequencing. The knowledge obtained from Raman metabolomic data can be used in drug discovery and pathogen intervention.PMID:33837016 | DOI:10.1128/AEM.00388-21
Source: Applied and Environmental Microbiology - Category: Microbiology Authors: Source Type: research