In Vivo Binding of Single-Stranded DNA-Binding Protein to Stalled Replication Fork Helicases

Methods Mol Biol. 2021;2281:81-91. doi: 10.1007/978-1-0716-1290-3_5.ABSTRACTUnderstanding protein-protein interactions is key to unraveling protein function in vivo. Here we describe a dual/triple-plasmid system that enables co-expression of two, or three, recombinant proteins harboring different affinity tags in the same Escherichia coli cell. This novel protein expression system provides a platform to understand protein-protein interactions and enables researchers to study protein complex formation and in vivo localization.PMID:33847953 | DOI:10.1007/978-1-0716-1290-3_5

Source: Mol Biol Cell - April 13, 2021 Category: Molecular Biology Authors: Cong Yu Piero R Bianco Source Type: research