Quantifying the Affinity of Trypanosoma cruzi RPA-1 to the Single-Stranded DNA Overhang of the Telomere Using Surface Plasmon Resonance

Methods Mol Biol. 2021;2281:217-228. doi: 10.1007/978-1-0716-1290-3_13.ABSTRACTSurface plasmon resonance (SPR) biosensors provide real-time binding affinity measurements between a pair of biomolecules, characterizing its interaction dynamics. An example of Trypanosoma cruzi's RPA-1 and a single-stranded DNA telomere sequence is presented with detailed guidelines and fundamentals for SPR technology.PMID:33847961 | DOI:10.1007/978-1-0716-1290-3_13
Source: Mol Biol Cell - Category: Molecular Biology Authors: Source Type: research