Reclassification of < em > Haloactinobacterium glacieicola < /em > as < em > Occultella glacieicola < /em > gen. nov., comb. nov., of < em > Haloactinobacterium album < /em > as < em > Ruania alba < /em > comb. nov, with an emended description of the genus < em > Ruania < /em > , recognition that the genus names < em > Haloactinobacterium < /em > and < em > Ruania < /em > are heterotypic synonyms and description of < em > Occultella aeris < /em > sp. nov., a halotolerant isolate from surface soil sampled at an ancient copper smelter

Int J Syst Evol Microbiol. 2021 Apr;71(4). doi: 10.1099/ijsem.0.004769.ABSTRACTIn the course of screening the surface soils of ancient copper mines and smelters (East Harz, Germany) an aerobic, non-motile and halotolerant actinobacterium forming small rods or cocci was isolated. The strain designated F300T developed creamy to yellow colonies on tryptone soy agar and grew optimally at 28 °C, pH 7-8 and with 0.5-2 % (m/v) NaCl. Its peptidoglycan was of type A4α l-Lys-l-Glu (A11.54). The menaquinone profile was dominated by MK-8(II, III-H4) and contained minor amounts of MK-8(H2), MK-8(H6) and MK-9(H4). The polar lipids comprised diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, mono and diacylated phosphatidylinositol dimannosides, and components that were not fully characterized, including two phospholipids, two glycolipids and an uncharacterized lipid. Major whole-cell sugars were rhamnose and ribose. The fatty acid profile contained mainly iso and anteiso branched fatty acids (anteiso-C15 : 0, iso-C14 : 0) and aldehydes/dimethylacetals (i.e. not fatty acids). Sequence analysis of its genomic DNA and subsequent analysis of the data placed the isolate in the group currently defined by members of the genera Ruania and Haloactinobacterium (family Ruaniaceae, order Micrococcales) as a sister taxon to the previously described species Haloactinobacterium glacieicola, sharing an average nucleotide identity and average amino acid identity values of 85.3 and 85.7 %...
Source: International Journal of Systematic and Evolutionary Microbiology - Category: Microbiology Authors: Source Type: research