The role of synonymous mutations in the evolution of TEM β-lactamase genes

Antimicrob Agents Chemother. 2021 Apr 5:AAC.00018-21. doi: 10.1128/AAC.00018-21. Online ahead of print.ABSTRACTNonsynonymous mutations are well documented in TEM β-lactamases. The resulting amino acid changes often alter the conferred phenotype from broad spectrum (2b) conferred by TEM-1 to extended spectrum (2be), inhibitor resistant (2br), or both extended spectrum and inhibitor resistant (2ber). The encoding bla TEM genes also deviate in numerous synonymous mutations, which are not well understood. bla TEM-3 (2be), bla TEM-33 (2br), and bla TEM-109 (2ber) were studied in comparison to bla TEM-1 bla TEM-33 was chosen for more detailed studies, because it deviates from bla TEM-1 by a single nonsynonymous mutation and three additional, synonymous mutations. Genes encoding the enzymes with only nonsynonymous or all, including synonymous, mutations plus all permutations between bla TEM-1 and bla TEM-33 were expressed in Escherichia coli cells. In disc diffusion assays, genes encoding TEM-3, TEM-33, and TEM-109 with all synonymous mutations resulted in higher resistance levels than genes without synonymous mutations. Disc diffusion assays with the 16 genes carrying all possible nucleotide change combinations between bla TEM-1 and bla TEM-33 indicated different susceptibilities for different variants. Nucleotide BLAST searches did not identify genes without synonymous mutations but some without nonsynonymous mutations. Energies of possible secondary mRNA structures calculated wi...
Source: Antimicrobial Agents and Chemotherapy - Category: Microbiology Authors: Source Type: research