Detection of extracellular superoxide in isolated human immune cells and in an animal model of arterial hypertension using hydropropidine probe and HPLC analysis

In conclusion, the HPr+/HPLC assay for superoxide determination is highly reliable in isolated immune cells and an animal model of arterial hypertension. In particular, the cell impermeability of HPr+ made it possible to differentiate between intra- and extracellular superoxide formation.PMID:33823245 | DOI:10.1016/j.freeradbiomed.2021.03.041

Source: Free Radical Biology and Medicine - April 6, 2021 Category: Biology Authors: Sanela Kalinovic Paul Stamm Matthias Oelze Sebastian Steven Swenja Kr öller-Schön Miroslava Kvandova Jacek Zielonka Thomas M ünzel Andreas Daiber Source Type: research