Conserved Plasmodium protein (PF3D7_0406000) of unknown function, in-silico analysis and cellular localization

Infect Genet Evol. 2021 Apr 3:104848. doi: 10.1016/j.meegid.2021.104848. Online ahead of print.ABSTRACTIn spite of a decrease in malaria cases, the threat of malaria due to Plasmodium falciparum still prevails. The sequencing of Plasmodium falciparum reveals that approximately 60% of the Plasmodium genes code for hypothetical/putative proteins. Here we report an in silico characterization and localization of one such protein. This was encoded by one of the hub genes, in a weighted gene co-expression based systems network, from in-vivo samples of patients suffering from uncomplicated malaria or complicated malaria disease like jaundice and jaundice with renal failure. Interestingly, the protein PF3D7_0406000 (PFD0300w) is classified as a conserved protein of unknown function and shows no identity with any protein from the human host. The transcriptomic data shows up-regulation of transcripts in cases of malaria induced disease complications. PFD0300w peptide antibody based immunolocalization studies using a, gametocyte producing P. falciparum strain RKL-9, shows presence of the protein in the cytoplasm of both asexual and sexual stage parasites.PMID:33823306 | DOI:10.1016/j.meegid.2021.104848
Source: Infection, Genetics and Evolution - Category: Genetics & Stem Cells Authors: Source Type: research