Current Advances in DNA Methylation Analysis Methods

Biomed Res Int. 2021 Mar 20;2021:8827516. doi: 10.1155/2021/8827516. eCollection 2021.ABSTRACTDNA methylation is one of the epigenetic changes, which plays a major role in regulating gene expression and, thus, many biological processes and diseases. There are several methods for determining the methylation of DNA samples. However, selecting the most appropriate method for answering biological questions appears to be a challenging task. The primary methods in DNA methylation focused on identifying the state of methylation of the examined genes and determining the total amount of 5-methyl cytosine. The study of DNA methylation at a large scale of genomic levels became possible following the use of microarray hybridization technology. The new generation of sequencing platforms now allows the preparation of genomic maps of DNA methylation at the single-open level. This review includes the majority of methods available to date, introducing the most widely used methods, the bisulfite treatment, biological identification, and chemical cutting along with their advantages and disadvantages. The techniques are then scrutinized according to their robustness, high throughput capabilities, and cost.PMID:33824878 | PMC:PMC8007345 | DOI:10.1155/2021/8827516

Source: Biomed Res - April 7, 2021 Category: Research Authors: Ehsan Khodadadi Leila Fahmideh Ehsaneh Khodadadi Sounkalo Dao Mehdi Yousefi Sepehr Taghizadeh Mohammad Asgharzadeh Bahman Yousefi Hossein Samadi Kafil Source Type: research