Deciphering the role of < em > ttrA < /em > and < em > pduA < /em > genes for < em > Salmonella enterica < /em > serovars in a chicken infection model

Avian Pathol. 2021 Mar 29:1-41. doi: 10.1080/03079457.2021.1909703. Online ahead of print.ABSTRACTSalmonella enterica serovars use intestinal self-induced inflammation to increase electron acceptor availability and to obtain a growth advantage in the host gut. There are evidences suggesting that the ability to use tetrathionate and 1,2-propanediol by Salmonella provides an advantage in murine infection. Thus, we present here the first study to evaluate both systemic infection and faecal excretion in commercial poultry challenged by Salmonella Enteritidis (SE) and S. Typhimurium (STM) harbouring deletions in ttrA and pduA genes, which are crucial to the metabolism of tetrathionate and 1,2-propanediol, respectively. Mutant strains were excreted at higher rates when compared to the wild-type strains. The highest rates were observed with white-egg layers and brown-egg layer chicks (67.5 %), and broiler chicks (56.7 %) challenged by SEΔttrAΔpduA, and brown-egg layers chicks (64.8 %) challenged by STMΔttrAΔpduA. SEΔttrAΔpduA presented higher bacterial counts in the liver and spleen of the three chicken lineages and caecal contents from the broiler chickens, whereas STMΔttrAΔpduA presented higher counts in the liver and spleen of the broiler and brown-egg chickens for 28 days challenge (P < 0.05). The ttrA and pduA genes do not appear to be major virulence determinants in faecal excretion or invasiveness for SE and STM in chickens.PMID:33779420 | DOI:10.1080/03079457.2021...
Source: Avian Pathology - Category: Pathology Authors: Source Type: research