Isolation and characterization of Campylobacter strains from diarrheal patients in Bangkok and its suburb in Thailand.

In this study, we attempted to isolate Campylobacter from diarrheal patients in Thailand and to characterize the species by a cytolethal distending toxin (cdt) gene-based species-specific multiplex PCR assay for C. jejuni, C. coli and C. fetus. The species of Campylobacters were also confirmed by 16S rRNA gene sequencing and detection of hipO gene. Of 2,500 stool specimens of diarrhea 76 Campylobacter-like organisms was isolated and identified by conventional culture methods. Among 76 campylobacters 73 were identified to be 43 C. jejuni, 29 C. coli, and 1 C. fetus, respectively, by the multiplex PCR assay, however 3 were unidentified. Two Campylobacter-like organisms yielded 2 amplicons corresponding to cdt genes of C. jejuni and C. coli. Subsequently C. jejuni and C. coli were re-isolated from each sample. The third isolate was identified as C. hyointestinalis by 16S rRNA gene sequencing. To our knowledge, this is the first report regarding the isolation of C. hyointestinalis from a diarrheal patient in Thailand. These data indicate that C. jejuni (58%) and C. coli (40%) are prevalent in diarrheal patients in Thailand. PMID: 25672405 [PubMed - as supplied by publisher]
Source: Japanese Journal of Infectious Diseases - Category: Infectious Diseases Authors: Tags: Jpn J Infect Dis Source Type: research