Photoinduced receptor confinement drives ligand-independent GPCR signaling

Cell-cell communication relies on the assembly of receptor-ligand complexes at the plasma membrane. The spatiotemporal receptor organization has a pivotal role in evoking cellular responses. We studied the clustering of heterotrimeric guanine nucleotide–binding protein (G protein)–coupled receptors (GPCRs) and established a photoinstructive matrix with ultrasmall lock-and-key interaction pairs to control lateral membrane organization of hormone neuropeptide Y2 receptors in living cells by light. Within seconds, receptor clustering was modulated in size, location, and density. After in situ confinement, changes in cellular morphology, motility, and calcium signaling revealed ligand-independent receptor activation. This approach may enhance the exploration of mechanisms in cell signaling and mechanotransduction.
Source: ScienceNOW - Category: Science Authors: Tags: Biochemistry, Cell Biology, Online Only r-articles Source Type: news

Related Links:

Source: BMJ Comments - Category: General Medicine Source Type: forums
Source: BMJ Comments - Category: General Medicine Source Type: forums
Source: BMJ Comments - Category: General Medicine Source Type: forums
OBJECTIVES: To describe mechanical ventilation management and factors associated with nonadherence to lung-protective ventilation principles in pediatric acute respiratory distress syndrome. DESIGN: A planned ancillary study to a prospective international observational study. Mechanical ventilation management (every 6 hr measurements) during pediatric acute respiratory distress syndrome days 0–3 was described and compared with Pediatric Acute Lung Injury Consensus Conference tidal volume recommendations (
Source: Critical Care Medicine - Category: Emergency Medicine Tags: Pediatric Critical Care Source Type: research
We examined whether the timing of performing quantitative pupillary light reflex or standard pupillary light reflex may impact its neuroprognostic performance in postcardiac arrest comatose patients and whether quantitative pupillary light reflex may outperform standard pupillary light reflex in early postresuscitation phase. DATA SOURCES: PubMed and Embase databases from their inception to July 2020. STUDY SELECTION: We selected studies providing sufficient data of prognostic values of standard pupillary light reflex or quantitative pupillary light reflex to predict neurologic outcomes in adult postcardiac a...
Source: Critical Care Medicine - Category: Emergency Medicine Tags: Review Articles Source Type: research
OBJECTIVES: To investigate whether intervention effect estimates for mortality differ between blinded and nonblinded randomized controlled trials conducted in critical care. We used a meta-epidemiological approach, comparing effect estimates between blinded and nonblinded randomized controlled trials for the same research question. DATA SOURCES: Systematic reviews and meta-analyses of randomized controlled trials evaluating a therapeutic intervention on mortality in critical care, published between January 2009 and March 2019 in high impact factor general medical or critical care journals and by Cochrane. DAT...
Source: Critical Care Medicine - Category: Emergency Medicine Tags: Review Articles Source Type: research
No abstract available
Source: Critical Care Medicine - Category: Emergency Medicine Tags: Editorials Source Type: research
No abstract available
Source: Critical Care Medicine - Category: Emergency Medicine Tags: Editorials Source Type: research
No abstract available
Source: Critical Care Medicine - Category: Emergency Medicine Tags: Editorials Source Type: research
No abstract available
Source: Critical Care Medicine - Category: Emergency Medicine Tags: Editorials Source Type: research
More News: Biochemistry | Biology | Brain | Calcium | Cytology | Hormones | Neurology | Science