Lipopolysaccharide Stimulates Bovine Endometrium Explants through Toll ‑like Receptor 4 Signaling and PGE2 Synthesis

Bovine endometrium infection with gram-negative bacteria commonly causes uterine diseases. Previous studies indicate that prostaglandin E2 (PGE2) is an inflammatory mediator in bacterial endometritis. However, the mechanism underlying lipopolysaccharide (LPS)-induced inflammatory response regulation in bovine endometrial explants remains elusive. In the present study, bovine explants were pre-treated with 15-hydroxyprostaglandin dehydrogenase (15-PGDH) inhibitors before LPS stimulation. PGE2 secretion, prostaglandin synthetase, pro-inflammatory factor, damage-associated molecular pattern (DAMP), and related signaling pathway factor levels were evaluated.

Source: Prostaglandins, Leukotrienes and Essential Fatty Acids - March 24, 2021 Category: Biomedical Science Authors: Yang Deng, Bo Liu, Changqi Fu, Long Gao, Yuan Shen, Kun Liu, Qianru Li, Jinshan Cao, Wei Mao Source Type: research