< em > Muricauda amphidinii < /em > sp. nov., a novel marine bacterium isolated from the phycosphere of dinoflagellate < em > Amphidinium carterae < /em >

Int J Syst Evol Microbiol. 2021 Mar 18. doi: 10.1099/ijsem.0.004764. Online ahead of print.ABSTRACTA Gram-stain-negative, aerobic, rod-shaped and non-motile bacterium was isolated from a liquid culture of dinoflagellate Amphidinium carterae and further designated as LMIT004T. Optimal growth was observed at 25 °C, pH 7.0 and in the presence of 2 % (w/v) NaCl. Oxidase and catalase were positive. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain LMIT004T showed high similarities to type strains Muricauda nanhaiensis SM17004T (96.77 %) and Muricauda aquimarina JCM11811T (95.60 %) but formed a separate branch in the genus Muricauda. The G+C content of strain LMIT004T was 39.0 mol%. The dominant fatty acids were iso-C15 : 0 and iso-C15 : 1 G. The polar lipids mainly contained phosphatidylethanolamine, five unidentified phospholipids and five unidentified polar lipids. The sole respiratory quinone was menaquinone-6 (MK-6). The draft genome of the type strain was 3.88 Mbp. The average nucleotide identity values between strain LMIT004T and the two reference strains M. nanhaiensis SM17004T and M. aquimarina JCM11811T were 77.47 and 73.49 %, respectively. Based on the polyphasic analysis, strain LMIT004T is suggested to represent a novel specie in the genus of Muricauda, for which the name Muricauda amphidinii sp. nov. is proposed. The type strain is LMIT004T (=CICC 24871T=KCTC 72948T).PMID:33734957 | DOI:10.1099/ijsem.0.004764
Source: International Journal of Systematic and Evolutionary Microbiology - Category: Microbiology Authors: Source Type: research