Nano-micelle packaging CRISPR/Cas9 components enables brain genome editing

(Innovation Center of NanoMedicine) The search of safe and efficient delivery methods of CRISPR/Cas9 is still ongoing. We fabricated nano-micelle co-loading Cas9 mRNA and gRNA in the core, and proved for the first time that RNA-based delivery of CRISPR/Cas9 induces genome editing in mouse brain parenchyma. This was possible because: first, co-loading gRNA with Cas9 mRNA protected gRNA from degradation, and second, polyethylene glycol-covered nano-micelle allowed better diffusion in brain tissue.

Source: EurekAlert! - Medicine and Health - March 10, 2021 Category: International Medicine & Public Health Source Type: news