Chemoenzymatic strategies for RNA modification and labeling

Curr Opin Chem Biol. 2021 Mar 6;63:46-56. doi: 10.1016/j.cbpa.2021.01.008. Online ahead of print.ABSTRACTRNA is a central molecule in numerous cellular processes, including transcription, translation, and regulation of gene expression. To reveal the numerous facets of RNA function and metabolism in cells, labeling has become indispensable and enables the visualization, isolation, characterization, and even quantification of certain RNA species. In this review, we will cover chemoenzymatic approaches for covalent RNA labeling. These approaches rely on an enzymatic step to introduce an RNA modification before conjugation with a label for detection or isolation. We start with in vitro manipulation of RNA, sorted according to the enzymatic reaction exploited. Then, metabolic approaches for co- and post-transcriptional RNA labeling will be treated. We focus on recent advances in the field and highlight the most relevant applications for cellular imaging, RNA isolation and sequencing.PMID:33690011 | DOI:10.1016/j.cbpa.2021.01.008
Source: Current Opinion in Chemical Biology - Category: Biochemistry Authors: Source Type: research