Temporal transcriptomes of a marine cyanopodovirus and its Synechococcus host during infection

Marine picocyanobacteria belonging to generaSynechococcus andProchlorococcus are genetically diverse and distributed into distinct biogeographical patterns, and both are infected by genetically closely related cyanopodoviruses. Here, we isolated and characterized a cyanopodovirus infectingSynechococcus, carried out RNA ‐seq analysis, and compared the results to the expression patterns of a cyanopodovirus and itsProchlorococcus host, which were previously reported. Our study demonstrated that cyanopodoviruses infecting marineSynechococcus andProchlorococcus undergo similar genome ‐wide expression dynamics and that gene regulations of the two host systems also respond similarly to infection. AbstractMarine picocyanobacteria belonging to generaSynechococcus andProchlorococcus are genetically diverged and distributed into distinct biogeographical patterns, and both are infected by genetically closely related cyanopodoviruses. Previous studies have not fully explored whether the two virus –host systems share similar gene expression patterns during infection. Whole‐genome expression dynamics of T7‐like cyanopodovirus P‐SSP7 and its hostProchlorococcus strain MED4 have already been reported. Here, we conducted genomic and transcriptomic analyses on T7 ‐like cyanopodovirus S‐SBP1 during its infection onSynechococcus strain WH7803. S ‐SBP1 has a latent period of 8 h and phage DNA production of 30 copies per cell. In terms of whole‐genome phylogenetic relationships...

Source: MicrobiologyOpen - February 16, 2021 Category: Microbiology Authors: Sijun Huang, Yingting Sun, Si Zhang, Lijuan Long Tags: ORIGINAL ARTICLE Source Type: research