Metabolic potential of the moderate halophile Yangia sp. ND199 for co ‐production of polyhydroxyalkanoates and exopolysaccharides

In this study, the potential of the bacterium for co ‐production of exopolysaccharides (EPS) and PHA was investigated. Genome sequence analysis of the closely relatedYangia sp. CCB ‐M3 isolated from mangroves in Malaysia revealed genes encoding enzymes participating in different EPS biosynthetic pathways. The effects of various cultivation parameters on the production of EPS and PHA were studied. The highest level of EPS (288 mg/L) was achieved using sucrose and yeast extrac t with 5% NaCl and 120 mM phosphate salts but with modest PHA accumulation (32% of cell dry weight, 1.3 g/L). Growth on fructose yielded the highest PHA concentration (85% of CDW, 3.3 g/L) at 90 mM phosphate and higher molecular weight EPS at 251 mg/L yield at 120 mM phosphate concentration. Analysi s of EPS showed a predominance of glucose, followed by fructose and galactose, and minor amounts of acidic sugars.

Source: MicrobiologyOpen - February 18, 2021 Category: Microbiology Authors: Luis Romero Soto, Habib Thabet, Reuben Maghembe, Denise Gameiro, Doan Van ‐Thuoc, Tarek Dishisha, Rajni Hatti‐Kaul Tags: ORIGINAL ARTICLE Source Type: research