Genes, Vol. 12, Pages 331: Analysis of Human Gut Microbiome: Taxonomy and Metabolic Functions in Thai Adults

Genes, Vol. 12, Pages 331: Analysis of Human Gut Microbiome: Taxonomy and Metabolic Functions in Thai Adults Genes doi: 10.3390/genes12030331 Authors: Nachon Raethong Massalin Nakphaichit Narissara Suratannon Witida Sathitkowitchai Wanlapa Weerapakorn Suttipun Keawsompong Wanwipa Vongsangnak The gut microbiome plays a major role in the maintenance of human health. Characterizing the taxonomy and metabolic functions of the human gut microbiome is necessary for enhancing health. Here, we analyzed the metagenomic sequencing, assembly and construction of a meta-gene catalogue of the human gut microbiome with the overall aim of investigating the taxonomy and metabolic functions of the gut microbiome in Thai adults. As a result, the integrative analysis of 16S rRNA gene and whole metagenome shotgun (WMGS) sequencing data revealed that the dominant gut bacterial families were Lachnospiraceae and Ruminococcaceae of the Firmicutes phylum. Consistently, across 3.8 million (M) genes annotated from 163.5 gigabases (Gb) of WMGS sequencing data, a significant number of genes associated with carbohydrate metabolism of the dominant bacterial families were identified. Further identification of bacterial community-wide metabolic functions promisingly highlighted the importance of Roseburia and Faecalibacterium involvement in central carbon metabolism, sugar utilization and metabolism towards butyrate biosynthesis. This work presents an initial study of shotgun metagenomics ...
Source: Genes - Category: Genetics & Stem Cells Authors: Tags: Article Source Type: research