Interactions of human mesenchymal stromal cells with peripheral blood mononuclear cells in a Mitogenic proliferation assay

CONCLUSIONS: A bidirectional interaction between MSCs and PBMCs occurs during co-culture. High numbers of MSCs inhibit PHA-stimulated PBMC proliferation and the PBMC response to stimulation; low numbers of MSCs augment these responses. Low density MSCs are susceptible to attrition, apparently by PBMC-induced apoptosis. These results may have direct application when considering therapeutic dosing of patients; low MSC doses may have unintended detrimental consequences.PMID:33609532 | DOI:10.1016/j.jim.2021.113000

Source: Journal of Immunological Methods - February 20, 2021 Category: Allergy & Immunology Authors: Maryanne C Herzig Barbara A Christy Robbie K Montgomery Christopher P Delavan Katherine J Jensen Sarah E Lovelace Carolina Cantu Christi L Salgado Andrew P Cap James A Bynum Source Type: research