GSE117053 Direct observation of CRISPR/Cas9 guide RNAs in single cell transcriptomes

Contributors : Yeo H Grace ; Juez Oscar ; Chen Qing ; Banerjee Budhaditya ; Chu Lendy ; Logister Ive ; Gifford K David ; Sherwood I RichardSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusWe introduce poly-adenine CRISPR gRNA-based single cell RNA-sequencing (pAC-Seq), a method enabling simultaneous observation of mutagenic guide RNAs (gRNAs) and their transcriptional consequences in single cell RNA sequencing (scRNA-seq) data. We have made gRNAs robustly visible in scRNA-seq data while maintaining full activity by modifying them with a hardcoded poly-adenine tract. We apply pAC-Seq to study the transcriptomic effects of non-coding CRISPR/Cas9-induced mutations in a pooled screening format. pAC-Seq is a simple, robust, and broadly applicable method of measuring the global effects of genomic mutation.

Source: GEO: Gene Expression Omnibus - February 24, 2021 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Mus musculus Source Type: research