Detection of < em > parC < /em > gene mutations associated with quinolone resistance in < em > Mycoplasma genitalium < /em > : evaluation of a multiplex real-time PCR assay

Conclusion. MG+parC(beta2) performed with high concordance compared to Sanger sequencing. Such qPCR assays can assist in understanding causes of treatment failure, inform the development of diagnostic assays, and can be applied to surveillance of mutations in populations. Due to an incomplete understanding of the basis for fluoroquinolone resistance, such tests do not appear to be ready for clinical application.PMID:33612146 | DOI:10.1099/jmm.0.001257

Source: Journal of Medical Microbiology - February 22, 2021 Category: Microbiology Authors: Kaveesha Bodiyabadu Jennifer Danielewski Suzanne M Garland Dorothy A Machalek Catriona S Bradshaw Joshua Birnie Samantha Ebeyan Marie Lundgren Gerald Murray Source Type: research