Genes, Vol. 12, Pages 307: Epigenetic Regulation of Processes Related to High Level of Fibroblast Growth Factor 21 in Obese Subjects

Genes, Vol. 12, Pages 307: Epigenetic Regulation of Processes Related to High Level of Fibroblast Growth Factor 21 in Obese Subjects Genes doi: 10.3390/genes12020307 Authors: Teresa Płatek Anna Polus Joanna Góralska Urszula Raźny Agnieszka Dziewońska Agnieszka Micek Aldona Dembińska-Kieć Bogdan Solnica Małgorzata Malczewska-Malec We hypothesised that epigenetics may play an important role in mediating fibroblast growth factor 21 (FGF21) resistance in obesity. We aimed to evaluate DNA methylation changes and miRNA pattern in obese subjects associated with high serum FGF21 levels. The study included 136 participants with BMI 27–45 kg/m2. Fasting FGF21, glucose, insulin, GIP, lipids, adipokines, miokines and cytokines were measured and compared in high serum FGF21 (n = 68) group to low FGF21 (n = 68) group. Human DNA Methylation Microarrays were analysed in leukocytes from each group (n = 16). Expression of miRNAs was evaluated using quantitative PCR-TLDA. The study identified differentially methylated genes in pathways related to glucose transport, insulin secretion and signalling, lipid transport and cellular metabolism, response to nutrient levels, thermogenesis, browning of adipose tissue and bone mineralisation. Additionally, it detected transcription factor genes regulating FGF21 and fibroblast growth factor receptor and vascular endothelial growth factor receptor pathways regulation. Increased expression of hsa-miR-875-5p and decreased exp...
Source: Genes - Category: Genetics & Stem Cells Authors: Tags: Article Source Type: research