Monocytes in Icelandic horses: comparison between automatic gating by the ADVIA 2120i, a manual gating method and microscopic differentiation

Abstract Inspection of the ADVIA 2120i PEROX cytograms from healthy Icelandic horses revealed a mismatch of the automatic gating (A) for monocytes, resulting in a lower percentage. The raw data of 40 Icelandic horses were transformed to FCS files. Data were imported to WinMDI 2.9 (Purdue Cytometry Laboratories) and gated manually (M). In addition, a manual 200-cell differential (D) was performed. ANOVA was used to test for significant differences. RefVal 2.1 (Toulouse Veterinary School) was used to calculate the reference values by different methods. Significance level was set at P 
Source: Comparative Clinical Pathology - Category: Pathology Source Type: research

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