Identification of PARP-1 in cancer stem cells of gastrointestinal cancers: A preliminary study.

Identification of PARP-1 in cancer stem cells of gastrointestinal cancers: A preliminary study. J Biosci. 2021;46: Authors: Quinonero F, Cepero A, Urbano D, Munoz-Gamez JA, Martin-Guerrero SM, Martin-Oliva D, Prados J, Melguizo C, Ortiz R Abstract Advanced-stage gastrointestinal tumors have high mortality due to chemotherapy limitations. One of the causes of treatment failure is the presence of cancer stem cells (CSCs), which show resistance mechanisms against DNA damage, such as poly (adenosine diphosphate-ribose) polymerase 1 (PARP-1). However, little is known about the relevance of PARP-1 in these tumor cells. Our purpose is to analyze the expression of PARP-1 in cancer cells and CSCs from gastrointestinal tumors, its relationship with the DNA damage repair process and its modulation by cytotoxic and PARP-1 inhibitors. We used pancreatic, liver and colon cancer cell lines and isolated CSCs using Aldefluor technology to analyze PARP-1 expression. In addition, we examined the effect of classic cytotoxic drugs (Doxorubicin, Gemcitabine, Irinotecan and 5-Fluorouracil) and a PARP-1 inhibitor (Olaparib) in cultured cells and 3D tumorspheres. We demonstrated that PARP-1 is highly expressed in pancreatic, liver and colon tumor cells and that this expression was significantly higher in cell populations with CSC characteristics. In addition, Doxorubicin and Gemcitabine increased their cytotoxic effect when administered simultaneously with O...
Source: Journal of Biosciences - Category: Biomedical Science Authors: Tags: J Biosci Source Type: research