Genotyping of RHD c.1227G > A allele by melting curve analysis

DEL is the weakest known D-positive phenotype and is detectable only by adsorption and elution tests. RHD c.1227G>A is an important marker for DEL phenotype in East Asians. The aim of this study was to develop a method for RHD c.1227G>A genotyping by single-tube PCR with melting curve analysis.

Source: Transfusion and Apheresis Science - February 5, 2021 Category: Hematology Authors: Ni Wang, Yaxin Fan, Shihang Zhou, Linnan Shao, Yuexin Xia, Ming Liu Source Type: research