Identification of Tick Ixodes ricinus Midgut Genes Differentially Expressed During the Transmission of Borrelia afzelii Spirochetes Using a Transcriptomic Approach

Lyme borreliosis is an emerging tick-borne disease caused by spirochetes Borrelia burgdorferi sensu lato. In Europe, Lyme borreliosis is predominantly caused by Borrelia afzelii and transmitted by Ixodes ricinus. Although Borrelia behavior throughout tick development is quite well documented, specific molecular interactions between Borrelia and the tick have not been satisfactorily examined. Here, we present the first transcriptomic study focused on the expression of tick midgut genes regulated by Borrelia. By using massive analysis of cDNA ends (MACE), we searched for tick transcripts expressed differentially in the midgut of unfed, 24h-fed, and fully fed I. ricinus nymphs infected with B. afzelii. In total, we identified 553 upregulated and 530 downregulated tick genes and demonstrated that B. afzelii interacts intensively with the tick. Technical and biological validations confirmed the accuracy of the transcriptome. The expression of five validated tick genes was silenced by RNA interference. Silencing of the uncharacterized protein (GXP_Contig_30818) delayed the infection progress and decreased infection prevalence in the target mice tissues. Silencing of other genes did not significantly affect tick feeding nor the transmission of B. afzelii, suggesting a possible role of these genes rather in Borrelia acquisition or persistence in ticks. Identification of genes and proteins exploited by Borrelia during transmission and establishment in a tick could help the develo...
Source: Frontiers in Immunology - Category: Allergy & Immunology Source Type: research

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Source: Herpes - Category: Infectious Diseases Authors: Source Type: research
The Borrelia burgdorferi sensu lato (s.l.) genogroup is the causative agent responsible for Lyme borreliosis, a common tick-borne infectious disease in some temperate regions of the Northern Hemisphere. In humans...
Source: Parasites and Vectors - Category: Microbiology Authors: Tags: Research Source Type: research
is the most common tick-borne illness in the USA and Europe. Pathogens involved include Borrelia burgdorferi in the USA and B. afzelii and B. garinii in Europe. The characteristic rash of erythema migrans occurs in 70 –80% of patients. Neurological disease, including facial palsy, meningo-encephalitis, aseptic meningitis and polyradiculopathy, occurs in 10–15%. Cardiac disease, primarily manifest as heart block, is seen in 1–4%. Arthritis is a late complication in about 30% of untreated patients.
Source: Medicine - Category: Internal Medicine Authors: Tags: Bacterial infections Source Type: research
Radiologia (Engl Ed). 2021 Sep-Oct;63(5):425-435. doi: 10.1016/j.rxeng.2021.07.002.ABSTRACTInfections of the central nervous system caused by atypical bacteria are becoming more common. Borrelia burgdorferi and Rickettsia conorii are microorganisms transmitted by ticks; infection with these bacteria result in a wide spectrum of manifestations on imaging. In areas where these tick-borne microorganisms are endemic, including Spain, these infections must be included in the differential diagnosis of patients with a variety of systemic and neurologic symptoms. The clinical presentation of these infections is nonspecific, and CT...
Source: Radiologia - Category: Radiology Authors: Source Type: research
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