Subunits of the GPI transamidase complex localize to the endoplasmic reticulum and nuclear envelope in Drosophila

AbstractA total of 10 –20% of plasma membrane proteins are anchored by glycosylphosphatidylinositol (GPI). GPI is attached to proteins by GPI transamidase (GPI‐T), which contains five subunits named PIGK, PIGS, PIGT, PIGU, and GPAA1. We previously reported that PIGT localizes near the nucleus inDrosophila. However, localizations of the other four subunits remain unknown. Here, we show that a catalytic subunit of GPI ‐T, PIGK, mainly localizes to the endoplasmic reticulum (ER), while the other four subunits localize to the nuclear envelope (NE) and ER. The NE/ER localization ratio of PIGS differs between cell types and developmental stages. Our results suggest that GPI‐T catalyzes GPI attachment in the ER an d the other four subunits may have other unknown functions in the NE.

Source: FEBS Letters - January 28, 2021 Category: Biochemistry Authors: Kohei Kawaguchi, Miki Yamamoto ‐Hino, Nina Matsuyama, Emiko Suzuki, Satoshi Goto Tags: RESEARCH LETTER Source Type: research