Whole blood can be used as an alternative to isolated peripheral blood mononuclear cells to measure in vitro specific T-cell responses in human samples.

In conclusion, we demonstrated that WB and PBMC methods of sample collection led to statistically concordant ICS results, indicating that WB-ICS is a suitable alternative to PBMC-ICS to analyze clinical trial samples.
PMID: 33493551 [PubMed - as supplied by publisher]

Source: Journal of Immunological Methods - January 22, 2021 Category: Allergy & Immunology Authors: Moris P, Bellanger A, Ofori-Anyinam O, Jongert E, Rodriguez JY, Janssens M Tags: J Immunol Methods Source Type: research