Towards defining the outer membrane proteome of Porphyromonas gingivalis

Porphyromonas gingivalis cell showing different types of outer membrane ‐associated proteins with their localization and function. AbstractPorphyromonas gingivalis is a Gram ‐negative anaerobic pathogen found in subgingival plaque associated with progressive periodontitis. Proteins associated with the outer membrane (OM) of Gram‐negative pathogens are particularly important for understanding virulence and for developing vaccines. The aim of this study was to establi sh a reliable list of outer membrane associated proteins (Omps) for this organism. Starting with a list of 99 experimentally determined Omps, several bioinformatics tools were used to predict a further 52 proteins, leading to a predicted OM proteome of 151 proteins. The tools used included databases of protein families, prediction of OM β‐barrels and structural homology. The list includes 33 T9SS cargo proteins, 43 lipoproteins and 66 OM β‐barrel proteins with some overlap between categories. The proteins are discussed both in these structural categories as well as their various function s in OM biogenesis, nutrient acquisition, protein secretion, adhesion and efflux. Proteins that were previously shown to be part of large complexes are highlighted and cross reference is provided to a previous major study of protein localization inP. gingivalis. Finally, proteins were also scored according to their level of conservation within theBacteroidales taxon. Low scores were shown to correlate with virulence f...
Source: Molecular Oral Microbiology - Category: Microbiology Authors: Tags: ORIGINAL ARTICLE Source Type: research