Evaluation of the expression of hexokinase 1, glucokinase, and insulin by canine insulinoma cells maintained in short-term culture.

CONCLUSIONS AND CLINICAL RELEVANCE: Canine insulinomas expressed hexokinases responsible for glucose responsiveness. Insulinoma cells were successfully maintained in short-term culture; cultured cells remained functional for 10 weeks as evidenced by cellular insulin content and had detectable secretion of insulin into the culture medium for ≥ 5 weeks. Apparent glucokinase overexpression by insulinomas suggested a possible mechanism underlying excessive insulin release by these tumors. PMID: 33480281 [PubMed - in process]
Source: American Journal of Veterinary Research - Category: Veterinary Research Authors: Tags: Am J Vet Res Source Type: research