Responsive Emulsions for Sequential Multienzyme Cascades.

Responsive Emulsions for Sequential Multienzyme Cascades.
Angew Chem Int Ed Engl. 2021 Jan 21;:
Authors: Sun Z, Zhao Q, Haag R, Wu C
Abstract
Multienzyme cascade biocatalysis is an efficient synthetic process, avoiding the isolation/purification of intermediates and shifting the equilibria of unfavourable steps. However, multienzyme systems are often limited by their incompatibility and cross-reactivity. Herein, we report a multi-responsive emulsion to proceed multienzyme reactions sequentially for high reactivity. The emulsion is achieved using a CO 2  , pH, and thermo-responsive block copolymer as a stabilizer, allowing the on-demand control of emulsion morphology and phase composition. Applying this system to a three-step cascade reaction enables the individual optimal condition for each enzyme, and a high overall conversion (ca. 97% of the calculated limit) is thereby obtained. Moreover, the multi-responsiveness of the emulsion allows the facile and separate yielding/recycling of products, polymers and active enzymes. Besides, the system could be scaled up with a good yield. Therefore, we present a recyclable, scalable, high reactive multienzyme biocatalysis using responsive emulsions, suggesting their fascinating perspectives for synthetic chemistry.
PMID: 33480131 [PubMed - as supplied by publisher]

Source: Angewandte Chemie - January 21, 2021 Category: Chemistry Authors: Sun Z, Zhao Q, Haag R, Wu C Tags: Angew Chem Int Ed Engl Source Type: research