Organelle-Specific Photoactivation of DNA Nanosensors for Precise Profiling of Subcellular Enzymatic Activity.

Organelle-Specific Photoactivation of DNA Nanosensors for Precise Profiling of Subcellular Enzymatic Activity. Angew Chem Int Ed Engl. 2021 Jan 22;: Authors: Shao Y, Zhao J, Yuan J, Zhao Y, Li L Abstract Design of sensors with controllable localization for in situ imaging of subcellular levels of enzymatic activity is particularly challenging, despite the importance in understanding of their functions in diverse cellular processes. We introduce herein a spatiotemporally-controlled sensor technology that permits in situ localization and photoactivated imaging of human apurinic/apyrimidinic endonuclease 1 (APE1) within an intracellular organelle of choice (e.g., mitochondria and nucleus). The hybrid sensor platform is constructed by photoactivatable engineering of a DNA-based fluorescent probe and further combination with an upconversion nanoparticle and a specific organelle localization signal. Controlled localization and NIR light-mediated photoactivation of the sensor "on demand" effectively constrains the imaging signal to the organelle of interest, with improved subcellular resolution. We further demonstrate the application of the nanosensors for the imaging of subcellular APE1 translocation in response to oxygen stress in live cells. Moreover, the combination of organelle-specific targeting with the remotely-controlled photoactivation of the nanosensors allows the precise APE1 imaging in vivo. We envision that this site-specific ...
Source: Angewandte Chemie - Category: Chemistry Authors: Tags: Angew Chem Int Ed Engl Source Type: research