Molecular Methods for Detection of Beta and Gammapapillomaviruses for Non-melanoma Skin Cancer

AbstractPurpose of reviewHuman Papillomaviruses (HPV) have some associations with that of the beta and gamma genera with non melanoma skin cancers (NMSC). The detection of HPV in clinical specimens is molecular-based since the isolation of the virus in in vitro cell lines has not been achieved. In this contest, we provide information about the choices of molecular methods applicable in epidemiological surveys.Recent findingsA limited number of assays have been developed to detect and genotype cutaneous HPVs (associated with NMSC). Consensus PCR followed by hybridization, consensus PCR followed by sequencing, multiplex PCR, and new genotyping are some methods to detect a proportion of the HPV types implicated in skin lesions with differing sensitivities and specificities.SummaryThe current review discusses about various choices on different tests in the detection of cutaneous HPVs. Most available methods rely on the amplification of DNA by consensus PCR followed by sequencing. Amplification of DNA by consensus PCR followed by sequencing assays is a powerful highthroughput tool that 24 could be used to detect multiple infections and to learn more about the natural history of genus alpha and beta skin HPV types. 25 This method is applicable for large epidemiological studies that require high-throughput testing to analyze the role of these HPVs 26 in human disease.
Source: Current Dermatology Reports - Category: Dermatology Source Type: research