Viruses, Vol. 13, Pages 113: Intranasal Infection of Ferrets with SARS-CoV-2 as a Model for Asymptomatic Human Infection
Viruses, Vol. 13, Pages 113: Intranasal Infection of Ferrets with SARS-CoV-2 as a Model for Asymptomatic Human Infection
Viruses doi: 10.3390/v13010113
Authors:
Helen E. Everett
Fabian Z.X. Lean
Alexander M.P. Byrne
Pauline M. van Diemen
Shelley Rhodes
Joe James
Benjamin Mollett
Vivien J. Coward
Paul Skinner
Caroline J. Warren
Kevin R. Bewley
Samantha Watson
Shellene Hurley
Kathryn A. Ryan
Yper Hall
Hugh Simmons
Alejandro Núñez
Miles W. Carroll
Ian H. Brown
Sharon M. Brookes
Ferrets were experimentally inoculated with SARS-CoV-2 (severe acute respiratory syndrome (SARS)-related coronavirus 2) to assess infection dynamics and host response. During the resulting subclinical infection, viral RNA was monitored between 2 and 21 days post-inoculation (dpi), and reached a peak in the upper respiratory cavity between 4 and 6 dpi. Viral genomic sequence analysis in samples from three animals identified the Y453F nucleotide substitution relative to the inoculum. Viral RNA was also detected in environmental samples, specifically in swabs of ferret fur. Microscopy analysis revealed viral protein and RNA in upper respiratory tract tissues, notably in cells of the respiratory and olfactory mucosae of the nasal turbinates, including olfactory neuronal cells. Antibody responses to the spike and nucleoprotein were detected from 21 dpi, but virus-neutralizing activity was low. A second intranasal inoculation (re-exposure) of two ferrets after a 1...
Source: Viruses - Category: Virology Authors: Helen E. Everett Fabian Z.X. Lean Alexander M.P. Byrne Pauline M. van Diemen Shelley Rhodes Joe James Benjamin Mollett Vivien J. Coward Paul Skinner Caroline J. Warren Kevin R. Bewley Samantha Watson Shellene Hurley Kathryn A. Ryan Yper Hall Hugh Simmons Tags: Article Source Type: research
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