Decreased expression of adenosine receptor 2B confers cardiac protection against ischemia via restoring autophagic flux.

Decreased expression of adenosine receptor 2B confers cardiac protection against ischemia via restoring autophagic flux. Am J Transl Res. 2020;12(12):7995-8006 Authors: Zhan E, Cao W, Fan X, Zhang R, Du H, Xu Y, Li L, Dong N, Li S Abstract Adora2B (adenosine receptor 2B) has been reported as one of the key modulators during cardiac remodeling after acute myocardial infarction (AMI). However, the molecular mechanism involved has not been well investigated. Thus, our study aims to investigate whether Adora2B contributes to cardiac remodeling after AMI and its underlying mechanisms. Adenovirus harboring Adora2B or shAdora2B was injected in the border zone in a mouse model of AMI experimentally produced by permanent ligation of left anterior descending (LAD) coronary artery. Decreased Adora2B expression protected the cardiomyocytes from MI-induced autophagic flux obstacle, improved cardiac function, and reduced fibrosis after MI. Adora2B downregulation attenuated the accumulation of LC3-II and p62, which are autophagy substrate proteins. An adenovirus containing mRFP-GFP-LC3 showed that decreased expression of Adora2B restored the autophagic flux by enhancing autophagosome conversion to autophagolysosome. Also, Adora2B knockdown improved cardiomyocytes' survival and protected mitochondrial function of cardiomyocytes insulted with hypoxia. Notably, the effect of Adora2B on autophagy flux and cardiomyocyte protection could be mitigated by ...
Source: American Journal of Translational Research - Category: Research Tags: Am J Transl Res Source Type: research