High-Throughput COVID-19 Diagnostic Test that Detects Both Viral and Host Nucleic Acid
The rapid worldwide spread and impact of COVID-19 caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has created a need for accurate, reliable, and readily accessible testing on a massive scale.NIEHS researchers developed a massively paralleled multiplexed screening method using next generation sequencing (NGS). This method uses sample-specific barcoded indexes that detect both SARS-COV-2 virus and the host ’s transcriptional response to infection simultaneously. By matching existing laboratory protocols for PCR-based sample processing, this assay is easily incorporated into existing CLIA-certified facilities. This testing approach provides the capability for testing tens of thousands of patient samp les in a large bolus, allowing accurate and fast-turnaround SARS-CoV-2 testing capacity at population scale, and permits monitoring of at-risk individuals.NIEHSE-241-2020-0Reduction in reagents needed to perform a test, reducing test cost and bottleneck of critical reagents used during nucleic acid amplificationSimultaneously detect the pathogen and a host ’s transcriptional response to infection by the pathogenGene expression information from the donor can be used to predict disease severityDiagnostic test for detecting infectious organisms, including SARS-CoV-23427Jan 11, 2021Monday, January 11, 202163/116,031USNIEHSNIEHSViditaChoudhryBG 31 RM 4A29 MSC 247
31 CENTER DRBethesda20892MD301-594-4095vidita.choudhry@nih.govPh.D.NIH Office of Technology Transfe...
Source: NIH OTT Licensing Opportunities - Category: Research Authors: ott-admin Source Type: research
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