Complete genome sequence of a novel foveavirus isolated from Allium sativum L. in China

AbstractThe complete genome sequence of a novel foveavirus identified in garlic (Allium sativum L.) in China was determined using RNA-seq, reverse transcription polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends (RACE) PCR. The entire genomic RNA (GenBank accession MT981417) is 8748 nucleotides long excluding the 3 ’-terminal poly(A) tail and contains five open reading frames (ORFs). These ORFs encode the viral replicase, a triple gene block, and a coat protein. The virus was tentatively named “garlic yellow stripe associated virus” (GarYSaV). Pairwise comparisons of protein sequences show that GarYSaV e ncodes proteins that share less than 47% identity with those of other foveaviruses, suggesting that it represents a new species in the genus. Phylogenetic analysis of amino acid sequences of the replicase and CP confirm that GarYSaV is a member of the genusFoveavirus. To our knowledge, this is the first report of a foveavirus in a monocot plant.
Source: Archives of Virology - Category: Virology Source Type: research