Viruses, Vol. 13, Pages 100: Molecular Epidemiology of Enterovirus in Children with Central Nervous System Infections

Viruses, Vol. 13, Pages 100: Molecular Epidemiology of Enterovirus in Children with Central Nervous System Infections Viruses doi: 10.3390/v13010100 Authors: Lamprini Posnakoglou Elizabeth-Barbara Tatsi Panagiota Chatzichristou Tania Siahanidou Christina Kanaka-Gantenbein Vasiliki Syriopoulou Athanasios Michos Limited recent molecular epidemiology data are available for pediatric Central Nervous System (CNS) infections in Europe. The aim of this study was to investigate the molecular epidemiology of enterovirus (EV) involved in CNS infections in children. Cerebrospinal fluid (CSF) from children (0–16 years) with suspected meningitis–encephalitis (ME) who were hospitalized in the largest pediatric hospital of Greece from October 2017 to September 2020 was initially tested for 14 common pathogens using the multiplex PCR FilmArray® ME Panel (FA-ME). CSF samples positive for EV, as well as pharyngeal swabs and stools of the same children, were further genotyped employing Sanger sequencing. Of the 330 children tested with FA-ME, 75 (22.7%) were positive for EV and 50 different CSF samples were available for genotyping. The median age of children with EV CNS infection was 2 months (IQR: 1–60) and 44/75 (58.7%) of them were male. There was a seasonal distribution of EV CNS infections, with most cases detected between June and September (38/75, 50.7%). EV genotyping was successfully processed in 84/104 samples:...
Source: Viruses - Category: Virology Authors: Tags: Article Source Type: research