Intratracheally instillated diesel PM2.5 significantly altered the structure and composition of indigenous murine gut microbiota.

In this study, C57BL/6J mice were exposed to diesel PM2.5 for 14 days via intratracheal instillation, and colon tissues and feces were harvested for microbiota analysis. Using high-throughput sequencing technology, we observed that intratracheally instillated diesel PM2.5 significantly altered the gut microbiota diversity and community. At the phylum and genus levels, principal coordinate analysis (PCoA) and principal component analysis (PCA) indicated pronounced segregation of microbiota compositions, which were further confirmed by β diversity analysis. As the most affected phylum, Bacteroidetes was greatly diminished by diesel PM2.5. On the genus level, Escherichia, Parabacteroides, Akkermansia, and Oscillibacter were significantly elevated by diesel PM2.5 exposure. Our findings provided clear evidence that exposure to diesel PM2.5 via intratracheal instillation deteriorated the gastrointestinal (GI) tract and significantly altered the structure and composition of gut microbiota, which might subsequently contribute to the developmental abnormalities of inflammation, immunity and metabolism.
PMID: 33429322 [PubMed - as supplied by publisher]

Source: Ecotoxicology and Environmental Safety - January 8, 2021 Category: Environmental Health Authors: Liu Y, Wang T, Si B, Du H, Liu Y, Waqas A, Huang S, Zhao G, Chen S, Xu A Tags: Ecotoxicol Environ Saf Source Type: research