AP ‐1 and NF‐κB synergize to transcriptionally activate latent HIV upon T‐cell receptor activation

In this study, we determined the contribution of the transcription factors NF‐κB, NFAT, and AP‐1 in the reactivation of latent HI V following T‐cell receptor (TCR) activation using Jurkat T‐cell clones harboring single latent HIV proviruses. Our findings demonstrate that during reactivation from latency, NF‐κB enhances HIV transcription while NFAT inhibits it by competing with NF‐κB for overlapping binding sites on t he HIV long terminal repeat (LTR). We have also demonstrated for the first time the molecular contribution of AP‐1 in the reactivation of HIV from latency, whereby AP‐1 synergizes with NF‐κB to regulate HIV transcriptional elongation following TCR activation.

Source: FEBS Letters - January 9, 2021 Category: Biochemistry Authors: Joseph Hokello, Adhikarimayum Lakhikumar Sharma, Mudit Tyagi Tags: RESEARCH ARTICLES Source Type: research