Volatile organic compounds in a headspace sampling system and asthmatics sputum samples

This study aimed to
characterise the background signal generated from a headspace sampling system in a clinical site, to
evaluate intra- and inter-day variation of background VOC and to understand the impact of a sample
itself upon commonly reported background VOC using sputum headspace samples from severe asthmatics.
The headspace, in absence of a biological sample, was collected hourly from 11am to 3pm within a day
(time of clinical samples acquisition), and from Monday to Friday in a week, and analysed by thermal
desorption-gas chromatography-mass spectrometry (TD-GC-MS). Chemometric analysis identified 1120
features, 37 of which were present in at least the 80% of all the samples. The analyses of intra-
and inter-day background variations were performed on 13 of the most abundant features, ubiquitously
presen...

Source: Journal of Breath Research - January 12, 2021 Category: Respiratory Medicine Authors: Rosa Peltrini, Rebecca L Cordell, Wadah Ibrahim, Michael J Wilde, Dahlia Salman, Amisha Singapuri, Beverley Hargadon, Christopher E Brightling, C L Paul Thomas, Paul S Monks, Salman Siddiqui and on behalf of the EMBER consortium Source Type: research